JOHN BRIDGES
97 Foothill Lane
Berkeley CA 94705
(510) 123-4567

Objective: Research Associate position with a biotechnology firm / basic research lab focusing on immunology and product development.

HIGHLIGHTS OF QUALIFICATIONS
  • Highly inquisitive, creative and resourceful.
  • Excellent skills in communication and collaboration.
  • Skilled in all phases of hybridoma production.
  • Good working knowledge of immunology.
  • Excited by the challenge of research and experimentation.

RELEVANT SKILLS and ACCOMPLISHMENTS

Applied Research
  • Successfully developed new antibodies for use in breast cancer research & therapy:
    --experimented with antigen preparation and immunization routines, resulting in the desired immune response;
    --carefully monitored the antisera to ensure presence of desired B-cell population;
    --tailored screening strategies using ELISA, RIA and immunoblot techniques, to effectively isolate the desired hybridomas.
  • Developed, in collaboration with others, a novel assay which identifies the antibodies' ability to bind to live, intact tumor cells.
  • Delivered periodic presentations of results and works in progress, to staff of Cancer Research Institute.

Innovation/Exploration
  • Pursued unique opportunities for experimentation, for example:
    --researched and worked out procedures for in vitro immunization of human lymphocytes;
    --explored and experimented with hybridoma production, using lymph node cells of a cancer patient;
    --experimented to induce animals' immune system to respond to a weak antigen.

Lab Skills

    
  • SDS-PAGE Electrophoresis
  • Radiolabeling of Antibodies
  • Tissue Culture
  • Affinity Chromatography
  • Immunoblot Strip Assay
  • Hamster Egg Penetration Test
  • Electroblotting
  • Lyophilization
  • Isotyping

EMPLOYMENT HISTORY
1994-present Lab Technician SCHILLING CANCER RESEARCH INST., Berkeley
1990-94 Full time student US Santa Barbara
'93 summer Research Asst. UC Santa Barbara Biology Dept.
1989 Youth Counselor RAINBOW RIVER DAY CARE PROGRAM, Los Angeles
1988 Emergency Med Tech SEALS AMBULANCE, Costa Mesa
1987 Teaching Asst. ALTA VISTA ELEM. SCHOOL, Redondo Beach

EDUCATION

B.A., Cell Biology & Physiology - UNIVERSITY OF CALIFORNIA, SANTA BARBARA, 1994
Related coursework: Immunology & Lab, Biochemistry, Virology, Microbiology